Aneuploid abortion correlates positively with MAD1 overexpression and miR-125b down-regulation

Abstract Background Aneuploidy is the most frequent cause of early-embryo abortion. Any defect in chromosome segregation would fail to satisfy spindle assembly checkpoint (SAC) during mitosis, halting metaphase and causing aneuploidy. The mitotic complex (MCC), comprising MAD1, MAD2, Cdc20, BUBR1 BUB3, plays a vital role SAC activation. Studies have confirmed that overexpression MAD2 can facilitate correct embryo stability. Research also proves miR-125b negatively regulates MAD1 expression by binding its 3′UTR. However, miR-125b, Mad1 Bub3 gene aneuploid embryos spontaneous abortion has not been reported date. Methods In this study, embryonic villi from miscarried pregnancies were collected divided into two groups (aneuploidy euploidy) based on High-throughput ligation-dependent probe amplification (HLPA) Fluorescence situ hybridization (FISH) analyses. RNA levels BUB3 detected Quantitative real-time PCR (qRT-PCR); protein analysed Western blotting. Results statistical analysis ( p < 0.05) showed significantly down-regulated aneuploidy group compared control was up-regulated. Additionally, level higher villus, but only mildly increased. Correlation revealed correlated with miR-125b. Conclusion These results suggest correlates positively overexpression, which might be caused insufficient Taken together, our findings first negative regulatory mode between providing basis for further mechanism researches